RealTime ready Cell Lysis Kit   實(shí)時(shí)準(zhǔn)備細(xì)胞 裂解試劑盒

Reagent for lysing cells prior to two-step real-time RT-PCR applications.

用于兩步實(shí)時(shí)RT-PCR應(yīng)用前裂解細(xì)胞的試劑。

For life science research only. Not for use in diagnostic procedures.

僅供生命科學(xué)研究使用。不用于診斷程序。

Overview概述：

Increase convenience:

Replace cumbersome multi-step RNA purification protocols with a simple one-step cell lysis procedure.

增加便利性：

用簡(jiǎn)單的一步細(xì)胞裂解程序取代繁瑣的多步 RNA 純化程序。

Improve time to result:

Lyse samples in just 5 minutes at room temperature without further steps.

縮短獲得結(jié)果的時(shí)間：

在室溫下僅需 5 分鐘即可裂解樣品，無(wú)需進(jìn)一步操作。

Reduce intermediate steps:

Obtain faster, high-quality results by performing DNase treatment during cDNA synthesis.

減少中間步驟：

通過(guò)在 cDNA 合成過(guò)程中進(jìn)行 DNase 處理，獲得更快、高質(zhì)量的結(jié)果。

Benefit from broad applicability:

Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats with or without automation.

受益于廣泛的適用性：

在 96 至 1536 孔格式的實(shí)時(shí) PCR 應(yīng)用中，無(wú)論是否采用自動(dòng)化，都可以將 3 到 30,000 個(gè)培養(yǎng)細(xì)胞放大。

Contents 內(nèi)容

1. Ready-to-use Lysis Buffer (20 mL)

   即用型裂解緩沖液 (20 mL)

• Protector RNase Inhibitor (250 µL - 40 U/µL)

   保護(hù)器核糖核酸酶抑制劑 (250 µL - 40 U/µL) 

• Thermolabile Nuclease (250 µL)

   耐熱核酸酶 (250 µL)

Application應(yīng)用：

The RealTime ready Cell Lysis Kit is designed for rapid, high-performance gene expression studies using cultured cells in two-step, real-time RT-PCR applications. The kit uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly in cDNA synthesis, eliminating the time-consuming RNA purification step. Finally, the cDNA can be analyzed in real-time PCR experiments at any throughput (e.g., LightCycler® 480 Instrument, 96- and 384-well; LightCycler® 1536 Instrument, 1536-well). Due to the user-friendly packaging and easy-to-use protocol, the RealTime ready Cell Lysis Kit can be easily adapted to all experimental and throughput requirements. In addition, the kit easily integrates into all manual and automated cell-based gene expression workflows.

實(shí)時(shí)準(zhǔn)備細(xì)胞裂解試劑盒設(shè)計(jì)用于在兩步實(shí)時(shí) RT-PCR 應(yīng)用中使用培養(yǎng)細(xì)胞進(jìn)行快速、高性能的基因表達(dá)研究。該試劑盒采用*一步式方案，可在室溫下 5 分鐘內(nèi)裂解 3 至 30,000 個(gè)細(xì)胞。所得裂解物可直接用于 cDNA 合成，省去了耗時(shí)的 RNA 純化步驟。最后，可以在任何通量的實(shí)時(shí) PCR 實(shí)驗(yàn)中分析 cDNA（例如，  LightCycler ®  480 儀器，96 孔和 384 孔；LightCycler ® 1536 儀器，1536 孔）。由于用戶友好的包裝和易于使用的協(xié)議，RealTime ready Cell Lysis Kit 可以輕松適應(yīng)所有實(shí)驗(yàn)和通量要求。此外，該試劑盒可輕松集成到所有手動(dòng)和自動(dòng)基于細(xì)胞的基因表達(dá)工作流程中。